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pcxb ebna1  (Addgene inc)


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    Structured Review

    Addgene inc pcxb ebna1
    Pcxb Ebna1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcxb+ebna1/pCXB-EBNA1+(Plasmid+%2341857)/pmc12946753-479-23-24
    Average 93 stars, based on 44 article reviews
    pcxb ebna1 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Transfection:

    Article Title: Multi-omic analysis of guided and unguided forebrain organoids reveals differences in cellular composition and metabolic profiles.
    Article Snippet: .. 86 Approximately 1 million PBMCs per line were transfected with 1 μg/μL in the dilution ratio 1:1:1:1 of pCXLE-hOCT3/4-shp53 (Addgene) pCXLE-hSK (Addgene), pCXLE-hUL (Addgene) pCXB-EBNA1 (Addgene) using the P3 Primary Cell 4D X Nucleofector Kit L and the Nucleofector Lonza Machine (BioNordika). ..

    Article Title: Multi-omic analysis of guided and unguided forebrain organoids reveals differences in cellular composition and metabolic profiles
    Article Snippet: .. Approximately 1 million PBMCs per line were transfected with 1 μg/μL in the dilution ratio 1:1:1:1 of pCXLE-hOCT3/4-shp53 (Addgene) pCXLE-hSK (Addgene), pCXLE-hUL (Addgene) pCXB-EBNA1 (Addgene) using the P3 Primary Cell 4D X Nucleofector Kit L and the Nucleofector Lonza Machine (BioNordika). .. Approximately 1 million PBMCs per line were transfected with 1 μg/μL in the dilution ratio 1:1:1:1 of pCXLE-hOCT3/4-shp53 (Addgene) pCXLE-hSK (Addgene), pCXLE-hUL (Addgene) pCXB-EBNA1 (Addgene) using the P3 Primary Cell 4D X Nucleofector Kit L and the Nucleofector Lonza Machine (BioNordika).

    Expressing:

    Article Title: Rapid and scalable personalized ASO screening in patient-derived organoids.
    Article Snippet: Cryopreserved PBMCs were thawed and cultured in StemSpan SFEM II (9605, StemCell Tech) supplemented with StemSpan erythroid expansion supplement (2692, StemCell Tech) and 1X antibiotic–antimycotic (15240062, Gibco) for 2–5 days before reprogramming using episomal plasmids. .. Episomal plasmids expressing OCT3/4, SOX2, KLF4, L-MYC, LIN28, p53 and EBNA1 (pCE-mP53DD (41856), pCXB-EBNA1 (41857), pCE-HUL (41855), pCE-hsk (41814), pCE-hOCT3/4 (41813; all Addgene) were introduced into PBMCs by electroporation using the Lonza 4D-Nucleofecter. .. The nucleofected PBMCs were transferred to a Matrigel (354277, Corning)-coated plate and maintained in StemSpan SFEM II plus 10 μM ROCK inhibitor.

    Article Title: Rapid and scalable personalized ASO screening in patient-derived organoids
    Article Snippet: Cryopreserved PBMCs were thawed and cultured in StemSpan SFEM II (9605, StemCell Tech) supplemented with StemSpan erythroid expansion supplement (2692, StemCell Tech) and 1X antibiotic–antimycotic (15240062, Gibco) for 2–5 days before reprogramming using episomal plasmids. .. Episomal plasmids expressing OCT3/4, SOX2, KLF4, L-MYC, LIN28, p53 and EBNA1 (pCE-mP53DD (41856), pCXB-EBNA1 (41857), pCE-HUL (41855), pCE-hsk (41814), pCE-hOCT3/4 (41813; all Addgene) were introduced into PBMCs by electroporation using the Lonza 4D-Nucleofecter. .. The nucleofected PBMCs were transferred to a Matrigel (354277, Corning)-coated plate and maintained in StemSpan SFEM II plus 10 μM ROCK inhibitor.

    Electroporation:

    Article Title: Rapid and scalable personalized ASO screening in patient-derived organoids.
    Article Snippet: Cryopreserved PBMCs were thawed and cultured in StemSpan SFEM II (9605, StemCell Tech) supplemented with StemSpan erythroid expansion supplement (2692, StemCell Tech) and 1X antibiotic–antimycotic (15240062, Gibco) for 2–5 days before reprogramming using episomal plasmids. .. Episomal plasmids expressing OCT3/4, SOX2, KLF4, L-MYC, LIN28, p53 and EBNA1 (pCE-mP53DD (41856), pCXB-EBNA1 (41857), pCE-HUL (41855), pCE-hsk (41814), pCE-hOCT3/4 (41813; all Addgene) were introduced into PBMCs by electroporation using the Lonza 4D-Nucleofecter. .. The nucleofected PBMCs were transferred to a Matrigel (354277, Corning)-coated plate and maintained in StemSpan SFEM II plus 10 μM ROCK inhibitor.

    Article Title: Rapid and scalable personalized ASO screening in patient-derived organoids
    Article Snippet: Cryopreserved PBMCs were thawed and cultured in StemSpan SFEM II (9605, StemCell Tech) supplemented with StemSpan erythroid expansion supplement (2692, StemCell Tech) and 1X antibiotic–antimycotic (15240062, Gibco) for 2–5 days before reprogramming using episomal plasmids. .. Episomal plasmids expressing OCT3/4, SOX2, KLF4, L-MYC, LIN28, p53 and EBNA1 (pCE-mP53DD (41856), pCXB-EBNA1 (41857), pCE-HUL (41855), pCE-hsk (41814), pCE-hOCT3/4 (41813; all Addgene) were introduced into PBMCs by electroporation using the Lonza 4D-Nucleofecter. .. The nucleofected PBMCs were transferred to a Matrigel (354277, Corning)-coated plate and maintained in StemSpan SFEM II plus 10 μM ROCK inhibitor.



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